Fig 7.

DGC overexpression in PA103 or PAO1 does not inhibit surface pilus assembly. (A) WT PA103 or PAO1 overexpressing indicated DGCs (PA1120 and PA3702) was grown on plates with 0.2% arabinose. Bars represent the twitching zone diameter as measured by the subsurface stab assay. (B) WT PA103 or PAO1 overexpressing indicated DGCs (PA0169, PA1120, and PA3702) were grown on plates with or without 0.2% arabinose. Surface pilin was measured by ELISA. Bars indicate means ± SD of triplicate samples from three independent experiments.