Fig 5.
PknK and ATP concentrations are positive effectors of PknK-mediated inhibition of protein synthesis. (A) The effects of ATP concentrations on the ability of wild-type and mutant PknK1-1110 proteins to inhibit phoP-IVTT were determined by monitoring PhoP synthesis by immunoblotting with anti-His antibody. In the presence of 2 mM exogenous ATP, wild-type PknK1-1110 protein (left blot), but not the PknK1-1110K55M mutant protein (right blot), completely inhibited the synthesis of PhoP. (B) Effects of increasing concentrations of ATP in reaction mixtures containing 5 μg of PknK KD-GST (left blot) and of purified PknK KD-GST in reaction mixtures containing 1 mM ATP (right blot) on the efficiency of the phoP-IVTT reaction were determined as described for panel A.