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(A) In the Nichols Sea strain of T. pallidum subsp. pallidum, a 125-bp-long replacement sequence can be found in the TP0131 5′-flanking region, within TP0136, containing one DS for V3 (green) and one for V7 (orange). (B) PCR amplification of the genomic region encompassing the insertion shows an amplicon of higher size only when Nichols Sea genomic DNA is used as the template. Lane M, size markers (sizes are in bp). Arrow indicates amplicon position.
