Table 1.
Primers used in this study
| Primer | Sequence | Usea | Target |
|---|---|---|---|
| 5DF-S | 5′-CAGTCCAAGAAGCGGAAAAG | 1, 2 | TP0131 5′-flanking region |
| 5DF-As | 5′-AAACGCCAGCAGTTCCAGCG | 1, 2 | TP0131 5′-flanking region |
| TP0136Ins-S | 5′-ACACAACGGCTGCGAATACT | 1 | TP0131 5′-flanking region |
| TP0136Ins-As | 5′-ACCGACCGTGCCCCATACT | 1 | TP0131 5′-flanking region |
| TPrflp-S | 5′-TTTTGTGTGTGGGGAGGAGT | 1, 2 | TP0131 5′-flanking region |
| TPrflpAs | 5′-ATGGCTGGGGGTAGGGCTTGC | 1, 2 | TP0131 5′-flanking region |
| 3DF-S | 5′-ATTGCCGAGAGCATCTGGT | 1, 2 | TP0131 3′-flanking region |
| 3DF-As | 5′-CCGCTCTCCTTCCCATAAGA | 1, 2 | TP0131 3′-flanking region |
| 3DF-S1 | 5′-GCCTCATACCGACTGGGAG | 1, 2 | TP0131 3′-flanking region |
| 3DF-S2 | 5′-CCTGCTCCTGGTACGAAGT | 1, 2 | TP0131 3′-flanking region |
| 3DF-S3 | 5′-CAGCAGGAACTCCCAGCACGT | 2 | TP0131 3′-flanking region |
| 3DF-As1 | 5′-GGGCGGACGTCCCACACC | 2 | TP0131 3′-flanking region |
| 3′DF-1-2-AS | 5′-ATTGCGTCTTTTGCGCGCG | 2 | TP0131 3′-flanking region |
| 3′DF-1-3-AS | 5′-AAACGCGGCGCTGTGGTTT | 2 | TP0131 3′-flanking region |
| 3′DF-1-4-AS | 5′-GGGAGTTGGGCTCGGGC | 2 | TP0131 3′-flanking region |
| 3′DF-1-5-AS | 5′-CTCGGGCTTGGGTGAAGG | 2 | TP0131 3′-flanking region |
| 3′DF-1-6-AS | 5′-ACACCCTGGGTGAAAAGACT | 2 | TP0131 3′-flanking region |
| 3′DF-1-7-AS | 5′-GTACGTGCTGGGAGTTCCT | 2 | TP0131 3′-flanking region |
| 3′DF-1-8-AS | 5′-TGTGCGGTCATGGCCATGT | 2 | TP0131 3′-flanking region |
| 3′DF-1-9-S | 5′-CGCAGGCCAGCAATGTATTT | 2 | TP0131 3′-flanking region |
| 3′DF-1-10-AS | 5′-TTTGGTTTGCCCTCCCAG | 2 | TP0131 3′-flanking region |
| 3′DF-1-11-S | 5′-CAAGCAGGGAGGACGCGT | 2 | TP0131 3′-flanking region |
| 3′DF-1-12-AS | 5′-CTTCGGGAATCGAGTGTAGT | 2 | TP0131 3′-flanking region |
| 3′DF-1-13-S | 5′-TCGTACTGCGCGTCATCGA | 2 | TP0131 3′-flanking region |
| 3′DF-1-14-S | 5′-TGGACTATCCATTCCCCATC | 2 | TP0131 3′-flanking region |
| 3′DF-1-15-AS | 5′-GTTGGGCTGGGGGAAATAG | 2 | TP0131 3′-flanking region |
| 3′DF-1-16-S | 5′-TATCGCCCCAACGCACGC | 2 | TP0131 3′-flanking region |
| 3′DF-1-17-S | 5′-GACGGGGAGGGCAAGTCA | 2 | TP0131 3′-flanking region |
| 3′DF-1-18-S | 5′-TACATGCCCGTCCATTGGAA | 2 | TP0131 3′-flanking region |
| 3′DF-1-19-AS | 5′-CATGGCATTGGTGAGAAAGA | 2 | TP0131 3′-flanking region |
| 3′DF-1-20-S | 5′-CAAAGACAGTCAGGGCAAGG | 2 | TP0131 3′-flanking region |
| 3′DF-1-21-AS | 5′-TGTCCTGTTCTTGTGCTCCA | 2 | TP0131 3′-flanking region |
| 3′DF-1-22-S | 5′-TCTACTTCTCGGCTCGCAGT | 2 | TP0131 3′-flanking region |
| 3′DF-1-23-AS | 5′-TATTGAGTGTGCGCTTACGG | 2 | TP0131 3′-flanking region |
| 3′DF-2-S | 5′-ACAACGAAGTTCCATAGGAC | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS | 5′-TTTAAAGGGTTCGTTCTCGC | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS-1 | 5′-ATCCTCTTACGCGTCTCGTC | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS-2 | 5′-CACTCCCGCGCATATTCCC | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS-3 | 5′-GCCGAAAGGCGTATAGTACT | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS-4 | 5′-TGCAAGGTAATAGTACGGAGT | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS-5 | 5′-TTGGATGGCGCATCGATTAC | 2 | TP0131 3′-flanking region |
| 3′DF-2-AS-6 | 5′-GCACGGCGCTGTTCAGTAA | 2 | TP0131 3′-flanking region |
| KI-S | 5′-GTGGTGTATCAGCGGGTAGG | 1, 2 | tprK ORF |
| KI-As | 5′-GACATGCCCCTACGAATTG | 1, 2 | tprK ORF |
| K-RFLP-S | 5′-TGGCGTGCAGGAATACATTA | 2 | tprK V3–V7 |
| K-RFLP-As | 5′-TACCCCACACTCGTAATACCC | 2 | tprK V3–V7 |
| K-RT-PCR-S | 5′-AGTTTGCGTCTAACACCGACTG | 2 | tprK V2–V5 |
| K-RT-PCR-As | 5′-TCGCATGGCCATGTTGAGAAAT | 2 | tprK V2–V5 |
| 9V-As | 5′-CCTCAAGGAAAGAAGTATCAGG | 2 | tprK V1–V5 |
| K-RACE-1 | 5′-AGCACACAGACCCCAAAGCTTC | 3 | tprK V3–V4 |
| K-RACE-2 | 5′-TAATGTATTCCTGCACGCCATC | 4 | tprK V4–V3 |
| K-RACE-3 | 5′-CAAACTTAAACGCAATATCAAA | 4 | tprK V2–V1 |
a
1, amplification (sequence analysis); 2, sequencing; 3, reverse transcription (5′-RACE protocol); 4, amplification (5′-RACE protocol).