Table 6.

G4FS identified in the Nichols strain genome of T. pallidum subsp. pallidum (19)

Position	Location	Length (bp)	Sequencea	G-score	Strand
12379b	TP0012	29	GGGAGCCTTCAATAAGGGGGAGGGAAGGG	32	+
32161	TP0025	17	GGGATAGGGGGGATGGG	39	+
72671	TP0067	25	GGGGGGGGGTATGGGGCTTATGGGG	59	+
82202	TP0074	30	GGGGAGTTGGGAACCCTTTTGGGAATTGGG	37	−
100635	TP0091	30	GGGCGGGGGTACCCCGGGTGGCACATCGGG	36	+
149328c	IGR104	22	GGGGCTGGGGCTGGGGCTGGGG	63	+
149398c	IGR104	26	GGGCTGCGGCTCCGGGTAGGGCTGGG	34	−
157297c	TP0136	29	GGGAACTGCGGTTGCGGGGGAGGGGGGGG	32	+
157393c	TP0136	29	GGGAACTGCGGTTGCGGGGGAGGGGGGGG	32	+
158101c	TP0136	29	GGGAACGGTGTCGCCGCGGGTGGGGCGGG	29	+
183960	TP0162	20	GGGTAAACGGGGGGAGTGGG	37	+
209260	TP0196	27	GGGAGCTGTAAGATGGGTCGGGGTGGG	33	+
213318	TP0207	27	GGGTCGTGGGTCCTCCTCTGGGCGGGG	35	+
230028	IGR175	20	GGGGGAGGGAAGGGTGTGGG	41	+
276466	TP0264	24	GGGTGCACAGGGTGAGGGAGGGGG	39	+
278453	IGR211	19	GGGAAGGGGGAGGGAAGGG	41	+
297175	TP0282	24	GGGGGGCTAGGGCTAAGTGCAGGG	33	+
316621	TP0303	29	GGGTAGAGGGAACAGGGATTCGTACCGGG	37	−
327048d	TP0312	25	GGGGGGAATAGTGGTGGGGATAGGG	33	+
327636d	TP0312	24	GGGAATGGGGATAGGGTTGCTGGG	40	+
327948d	IGR236	16	GGGCCGGGGGGGGGGG	41	+
346017	TP0326	27	GGGTGAACGGGGGGGTGGACTTTCGGG	34	+
357406	TP0334	27	GGGATTCGGGTGTGGGCATCTAACGGG	37	−
371115	TP0345	30	GGGAGGGCACCCCTTGCGGGGCACGCCGGG	33	+
371369	TP0346	27	GGGTATGGGCGCTTTGGGATATATGGG	39	+
433549	TP0408	26	GGGGGGCTGTGGGTCGGTCAAACGGG	32	+
441155	TP0414	25	GGGTGACGGGGATATTCGGGATGGG	38	+
464653	TP0438	19	GGGCGGGGGGTGTGCCGGG	36	+
485699	TP0456	30	GGGTGAGGGCGGTGCATTGGGCGTTCCGGG	36	+
485881	TP0456	20	GGGTGGGGGGAACTGTTGGG	35	+
488893	TP0458	26	GGGTGAACAGAGGGTGCAGGGGAGGG	36	−
489727	IGR335	24	GGGGGGAGGGTGACTGAAGGTGGG	31	+
521840	TP0488	26	GGGATTTTTCTGTAGGGGGGGTGGGG	32	+
540710	IGR364	24	GGGGGGAGGGTGTCGGAGGACGGG	31	+
742540	IGR483	30	GGGCGGGGTATGCGCAGGGTGCACAGCGGG	36	+
749636	TP0685	30	GGGAAAGGGTGCCGTGGTGGGGCTTATGGG	36	+
778932	TP0711	30	GGGCATCGGGTGCGGGGCAGGAGACTCGGG	36	−
798620	TP0733	23	GGGGCGGGGGGTTTCACCTCGGG	33	+
830276	IGR549	22	GGGCCTTTTTGAGGGCGGGGGG	33	+
933620	TP0856	30	GGGTACCGACGTGGGTCTGCAATGGGTGGG	34	+
936331	TP0859	27	GGGTATCGCCACACCCGGGGGGGGGGG	30	+
976267e	TP0898	22	GGGGGGACTGCGGGGTCAAGGG	37	−
977324e	TP0898	16	GGGGGGTGCGGGTGGG	39	+
986979	TP0905	25	GGGGGGCAGGGCGTCTAGGCAAGGG	31	+
1061766	TP0978	24	GGGATTATCGGGGGAGGGATAGGG	39	+
1123979f	TP1030	15	GGGCGGGGGGGGGGG	42	+

^aPutative G residues involved in formation of the G quadruplex are underlined.

^bPutative G4FS located downstream of tprB.

^cPutative G4FS located within the tprD (TP0131) 3′-flanking region (containing tprK DSs). These sequences were tested for ability to form G4 using circular dichroism.

^dPutative G4FS located upstream of tprE.

^ePutative G4FS located upstream of tprK expression site. These sequences were tested for ability to form G4 using circular dichroism.

^fPutative G4FS located upstream of tprL.