Fig 2.

Contribution of Rot and the Sae TCS to Ssl production in strain Newman. The indicated Newman strains were grown to early stationary phase under either inducing (+ hemin) or noninducing (− hemin) conditions. Exoproteins were collected, precipitated, separated using SDS-PAGE, either stained with Coomassie blue (top panels) or transferred to nitrocellulose, and immunoblotted for the indicated Ssls (bottom panel). Corresponding whole-cell lysates were also immunoblotted for Rot. The arrow indicates Ssls.