Fig 3.

Role of the Sae TCS in the activation of the ssl promoters. (A and B) The indicated Newman strains were transformed with plasmids containing the ssl7 or ssl9 promoter controlling GFP gene expression, and GFP fluorescence was monitored at the indicated time points. Values represent averages from three independent experiments ± standard deviations (SD). (C) Reporter assay as done for panel A. Closed symbols indicate wild-type promoters, and open symbols indicate promoters in which the SaeR binding site has been mutated to impair SaeR binding.