Table 3.
Plasmids
| Name | Description | Resistance | Reference or source |
|---|---|---|---|
| pOS1-PhrtAB | Empty vector control containing the hrtAB promoter | Cm | 51 |
| pOS1-PhrtAB-rot6 × His | hrtAB promoter controlling expression of rot with a C-terminal His tag | Cm | This study |
| pOS1-PhrtAB-saeRS | hrtAB promoter controlling expression of saeRS | Cm | This study |
| pOS1-Plgt | lgt promoter in an empty vector | Cm | 10 |
| pOS1-Plgt-saeS (Newman) | lgt promoter controlling expression of saeS amplified from strain Newman | Cm | This study |
| pOS1-Plgt-saeS (LAC) | lgt promoter controlling expression of saeS amplified from strain LAC | Cm | This study |
| pOS1-Plgt-rot3 × FLAG | lgt promoter controlling expression of rot containing a C-terminal FLAG tag | Cm | This study |
| pCM11 | sarA promoter and sod RBS controlling expression of sgfpa | Erm | 29 |
| pOS1sGFP promoterless | pOS1 containing the sgfp with no promoter | Cm | 7 |
| pOS1sGFP-Pssl7-sod RBS | ssl7 promoter controlling expression of sgfp | Cm | 7 |
| pOS1sGFP-Pssl9-sod RBS | ssl9 promoter controlling expression of sgfp | Cm | 7 |
| pOS1sGFP-Pssl11-sod RBS | ssl11 promoter controlling expression of sgfp | Cm | 7 |
| pOS1sGFP-PsarA-sod RBS | sarA promoter controlling expression of sgfp | Cm | This study |
| pOS1sGFP-Prot-sod RBS | rot promoter controlling expression of sgfp | Cm | This study |
| pOS1sGFP-PsaeP1 | sae P1 promoter controlling expression of sgfp | Cm | This study |
| pOS1sGFP-Pssl7-saeR-sod RBS | ssl7 promoter with mutated SaeR binding site controlling expression of sgfp | Cm | This study |
| pOS1sGFP-Pssl9-saeR-sod RBS | ssl9 promoter with mutated SaeR binding site controlling expression of sgfp | Cm | This study |
| pET41b | Empty expression vector with IPTG-inducible promoter | Amp | Novagen |
| pET41b saeSΔN92 | saeS deltaN92 expression vector | Amp | This study |
| pET41b saeR | saeR expression vector | Amp | This study |
| pET14b | Empty expression vector with IPTG-inducible promoter | Kan | Novagen |
| pET14b rot | rot expression vector | Kan | This study |
| pKOR1 | Empty vector used for allelic replacement | Cm | 5 |
| pKOR1 ΔsaeQRS::spec | pKOR1 vector containing saeQRS-homologous regions with aad insertion | Cm | This study |
a
sgfp, superfolder green fluorescent protein gene.