Fig 3.

RN4220 sigS mutants have a growth defect upon exit from stationary phase. Optical density readings (OD₆₀₀) of the RN4220 wild-type (■), sigS mutant (□), and sigS complement (✦) strains were taken every hour for 6 h and again at 24 h during growth at 37°C with shaking in TSB. Growth curves are representative of at least three independent experiments that showed less than 10% variability. *, P < 0.05 by the Student t test, indicating significant difference in growth between the sigS mutant and its parental and complemented strains.