Fig 8.

sigS mutants are sensitive to a number of cell wall-targeting antibiotics. The USA300 wild-type (W) and sigS mutant (M) strains were grown in TSB containing increasing concentrations of the cell wall-targeting antibiotics bacitracin, ampicillin, and penicillin G in a 96-well plate format. The cultures were allowed to grow overnight at 37°C and subsequently analyzed for growth, and the MICs were determined. The data are representative of at least three independent experiments that showed less than 10% variability. *, P < 0.05 by a Student t test.