. 2012 Aug;194(16):4272–4284. doi: 10.1128/JB.00409-12

Table 3.

yjiY induction is affected by mutations in the proposed phosphorylation sites of YehU/YehT and underlies cAMP/CRP regulation

Type of regulation and E. coli strain/reporter plasmid	Additional plasmid	Description of deletion or substitution	Maximal induction of yjiY (RLU/OD₆₀₀)^a
Type of regulation and E. coli strain/reporter plasmid	Additional plasmid	Description of deletion or substitution	LB	LB + 0.00006% Ara	LB + 0.2% Ara
YehU/YehT regulation
E. coli MG1655 rpsL150/pBBR yjiY-lux	pBAD24	Wild type	22,000	19,000	5,000
E. coli MG3/pBBR yjiY-lux	pBAD24	ΔyehT	1	1	1
E. coli MG3/pBBR yjiY-lux	pBAD24-yehT	yehT	23,000	20,000	170,000
E. coli MG3/pBBR yjiY-lux	pBAD24-yehT-D54E	yehT-D54E	39,000	87,000	200,000
E. coli MG3/pBBR yjiY-lux	pBAD24-yehT-D54N	yehT-D54N	2,200	17,000	170,000
E. coli MG6/pBBR yjiY-lux	pBAD24	ΔyehU	1	1	1
E. coli MG6/pBBR yjiY-lux	pBAD24-yehU	yehU	42,000	44,000	300
E. coli MG6/pBBR yjiY-lux	pBAD24-yehU-H382Q	yehU-H382Q	1	1	1
cAMP/CRP regulation
E. coli MG1655/pBBR yjiY-lux	None	Wild type	12,000	ND	ND
E. coli MG10/pBBR yjiY-lux	None	ΔyjiY	42,000	ND	ND
E. coli MG11/pBBR yjiY-lux	None	ΔcyaA	2,700	ND	ND
E. coli MG12/pBBR yjiY-lux	None	Δcrp	1	ND	ND
E. coli MG1655/pBBR yjiY-sub-CRP-BS-lux	None	Substitution of the CRP-binding site in P_yjiY	2,300	ND	ND

Strains were grown aerobically in a complex medium (LB) in the absence or presence of l-arabinose (Ara). Growth and luciferase activity were monitored continuously. The maximal luciferase activity normalized to an optical density of 1 (RLU/OD₆₀₀) was used as a measure of the degree of induction of yjiY. Data were obtained from at least three independent experiments, and average values (the standard deviation was less than 15%) were used for calculations. ND, not determined.