Fig 3.

Role HCoV-229E cell entry and replication on Mo-DC death. (A) Mo-DCs were incubated or not with anti-CD13 MAb prior to infection with HCoV-229E. Cell death was determined by the observation of cytopathic effects and the formation of syncytia by bright-field microscopy (the results of one representative experiment out of three are shown). (B) Mo-DCs were treated or not with increasing amounts of IFN-β prior to infection with HCoV-229E, and the percentages of cells expressing spike viral protein or activated caspase-3 were determined by flow cytometry (the results of one representative experiment out of three are shown).