Fig 6.

Interactions between the ToMV helicase domain and host proteins. (A) Start and stop positions of the ToMV-Hel deletion mutants used in the split-ubiquitin-based yeast two-hybrid assay. (B) Interactions between TOM1 and ToMV-Hel deletion mutants assessed by the split-ubiquitin-based yeast two-hybrid assay. (C) Interaction between ARL8 and ToMV-Hel deletion mutants assessed by the split-ubiquitin-based yeast two-hybrid assay. (D) Effect of the long α-helix from the ToMV-Hel N-terminal domain on the interaction of TOM1 with ARL8 as assayed by the split-ubiquitin-based yeast three-hybrid system. Averages and standard deviations of β-galactosidase activity (Miller units) for three or four independent yeast transformants are shown in panels B to D.