Table 1.
Distribution of HLA-restricted ELISpot responses in WNV proteinsa
| WNV protein | Protein size (aa)b | No. of overlapping peptides | No. (∼%) of ELISpot-positive peptides | HLA-restricted T-cell activation |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| A2 | A24 | B7 | DR2 | DR3 | DR4 | Total | ||||
| C | 123 | 15 | 1 (7) | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
| prM | 167 | 21 | 6 (29) | 1 | 2 | 1 | 6 | 3 | 4 | 17 |
| E | 501 | 67 | 25 (37) | 8 | 6 | 2 | 8 | 12 | 6 | 42 |
| NS1 | 352 | 46 | 10 (22) | 2 | 1 | 0 | 1 | 3 | 5 | 12 |
| NS2a | 231 | 30 | 10 (33) | 0 | 3 | 0 | 8 | 3 | 3 | 17 |
| NS2b | 131 | 16 | 6 (38) | 1 | 3 | 1 | 2 | 0 | 2 | 9 |
| NS3 | 619 | 84 | 46 (55) | 26 | 0 | 1 | 16 | 11 | 4 | 58 |
| NS4a | 149 | 18 | 5 (28) | 0 | 3 | 0 | 1 | 1 | 2 | 7 |
| NS4b | 255 | 33 | 9 (27) | 0 | 1 | 0 | 3 | 3 | 5 | 12 |
| NS5 | 905 | 122 | 19 (16) | 2 | 5 | 4 | 5 | 2 | 7 | 25 |
| Total | 3,433 | 452 | 137 (30) | 40 | 24 | 10 | 50 | 38 | 38 | 200 |
| Percentage of positive ligand sequences per complete proteome array | ∼9% | ∼5% | ∼2% | ∼11% | ∼8% | ∼8% | ||||
a
As identified by IFN-γ ELISpot assay in HLA Tg mice immunized with WNV overlapping peptides.
b
Corresponding to the WNV flamingo strain (NCBI Entrez Protein Database accession number AAF20092.2).