Fig 4.

Identification of the Nef 196-203 HW8 epitope. (A) MHC-matched BLCLs were pulsed with serial dilutions of prospective optimal peptides and cocultured with a Nef 196-203 HW8-specific T cell line. Activation of CD8⁺ T cells was measured by ICS using αIFN-γ-FITC and αTNF-α-PE. When responses were normalized to the percentage of the maximum response, Nef 196-203 HW8 was determined to be the optimal epitope. (B) MHC class I transferent K562 antigen-presenting cells were pulsed with cognate peptide and cocultured with a Nef 196-203 HW8-specific T cell line. Activation of CD8⁺ T cells was measured by ICS as described for panel A. Nef 196-203 HW8 was found to be restricted by Mafa-A1*063. Results are representative of at least two separate experiments.