Fig 6.

Deficient actin- and microtubule-based motility of ECTV ΔA36R. (A) HeLa cells were infected with ECTV or ECTV ΔA36R, fixed, and probed in immunofluorescence assays with anti-B5 (NPC) (green) and DAPI (blue). The dotted line shows the cell periphery as determined by phase-contrast microscopy. Infected cells were also probed with anti-B5 (NPC) (red), KHC antiserum (green), and DAPI (blue) (B) or with anti-B5 (NPC) (red), phalloidin (green), and DAPI (blue) (C). mRFP expressed by ECTV ΔA36R was bleached by the addition of a high concentration of p-phenylenediamine (1% [wt/vol]) to the mounting solution. Arrowheads indicate WV. Bars, 10 μm.