Fig 4.

Influenza virus-specific T cells coexpress CXCR5 and CXCR4. A total of 1 × 10⁶ MACS-enriched CD4⁺ T cells from TCR transgenic TS-1 mice, specific for HA of influenza virus A/PR8, were transferred into BALB/c mice (n = 4 per time point). Mice were infected immediately, and MedLN were analyzed by 9-color flow cytometry at 7 and 14 days postinfection. (A) Transgenic CD4⁺ T cells were identified by using a clonotype-specific MAb and analyzed for chemokine receptor expression as described in the legend of Fig. 2A. Shown are dot plots and 5% contour FACS plots with outliers of a representative sample for each time point. (B) Bar charts indicating mean frequencies ± SD of each CD4 T cell subset among TS-1 transgenic and endogenous cells. Data were pooled from two replicate experiments.