Fig 7.

Interactions of paramyxovirus V proteins with RIG-I, MDA5, IRF3, and IRF3-5D as revealed by IP-Western blotting. 293T cells were cotransfected with the indicated plasmids. After 24 h, cells were suspended in cell lysis buffer and then immunoprecipitated with anti-myc or anti-MuV P antibody to precipitate paramyxovirus V proteins. The immunoprecipitates were separated by SDS-PAGE, followed by Western blotting using anti-FLAG or anti-HA antibody to detect coprecipitated epitope-tagged host proteins, FLAG-MDA5, FLAG-RIG-I, HA-IRF3, and HA-IRF3-5D. Cell lysates were also subjected to Western blotting with individual antibodies to confirm expression of proteins. An experiment using a reverse combination of antibodies was also performed.