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Genome compositions of recombinant 7- and 8-segmented HEF viruses carrying a rewired PB1, HA, or NS segment. Using the same method as in Fig. 4, six HEF viruses, ΔPB1-PS (A), ΔPB1-PS+PB1-GFP-PB1 (B), ΔHA-PS (C), ΔHA-PS+HA-GFP-HA (D), ΔNS-PS (E), and ΔNS-PS+NS-GFP-NS (F), were analyzed for genome composition using an RNA gel and silver-staining method. The quantifications of the bands at the bottom of each gel are also shown. ?, unknown band.
