Fig 1.

Location of progeny virions in HSV-1-infected Vero cells. (A to D) Thin-section electron micrographs of infected Vero cells fixed and processed on coverslips at 12 hpi. Note that the majority of virions are released in pockets along the adherent cell surface (arrowheads) and at cell-cell contact points (arrows), with some virus above cell-cell contacts (bracket). Panel B is an enlargement of the left arrowhead in panel A; panel C is an enlargement of the right arrowhead in panel A. (E and F) Electron micrographs of mock-infected Vero cells. Note that, unlike infected cells, the cell membrane is closely apposed to the coverslip edge. In panels A to F the coverslip interface is marked by the thin line near the bottom surface of the cell. (G) Consecutive Z-stack confocal images of a representative glass coverslip-grown Vero cell infected with VP26-GFP mutant virus. Sections begin at the coverslip and go up by 0.25-μm increments. Asterisks mark the two sections above the coverslip where the majority of virions can be seen. Other sections have far fewer virions. The nucleus (containing VP26-GFP-labeled capsid assembly areas) is outlined. Arrows indicate virus released along a cell-cell contact. Images were obtained with a Philips 400T transmission electron microscope (A to F) and a Zeiss LSM 510 confocal microscope with an inverted 100× lens (G).