Fig 4.

Location of cytoskeletal elements in relation to viral egress sites on the coverslip-adherent surface of HSV-1-infected Vero cells as shown by TIRF microscopy. (A to C) VP26-GFP HSV-infected Vero cells stained with α-tubulin antibody to label microtubules (A), Texas Red-phalloidin (TR-Phalloidin) to stain actin (B), or α-vinculin antibody to mark focal adhesions (C). Note that areas where virions accumulate are depleted of all three cytoskeletal elements. The lined areas mark representative egress sites where the depletion has occurred. The starred area in panel B is an adjacent uninfected cell. (D to F) Mock-infected cells stained with α-tubulin antibody (D), Texas Red-phalloidin (E), or α-vinculin antibody (F). Note that large areas of cytoskeletal depletion are rarely seen in uninfected cells. Alexa 594-conjugated goat anti-mouse secondary antibody was used in panels A, C, D, and F. The size marker in panel F is relevant for all panels.