Fig 6.

TIRF micrographs showing egress site reformation after actin depolymerization-induced disruption. VP26-GFP-infected Vero cells at 11.5 hpi (A) were treated with 1.7 μg/ml cytochalasin B for 30 min (B). (C) Toxin was then rinsed out and the infection continued for another 45 min. Sample cells were fixed at each step and stained with Texas Red-phalloidin or rhodamine-WGA. (D) Untreated control. Note that both the actin cortex and viral egress sites were able to reorganize after disruption. There is no global block in actin polymerization.