Fig 7.

Western blot analysis of gE expression in deletion and rescue mutants. Cell lysates and supernatants of wild-type HSV- and gE mutant-infected Vero cells were collected at 18 hpi. Samples were run on an SDS-polyacrylamide gel, transferred, and blotted with α-gE antibody. The gE antibody used is against the extracellular domain of gE (25). α-VP5 was used as a loading control. Note that gE is expressed normally in the WT and rescue samples (gEΔCT.R) but not in the deletion mutant-infected samples. The arrowhead points to a nonspecific band. Truncated gE is indicated by a star.