Fig 8.

Effect of gE and gE cytoplasmic tail deletions on patch formation and progeny virion trafficking. (A) TIRF images of Vero cells infected with the ΔgE mutant, the gEΔCT mutant, or the gEΔCT rescue virus at an MOI of 0.3 for 12 h. Cells were fixed and treated with rhodamine-WGA and α-VP5 antibody with Alexa 488-conjugated secondary antibody. Note that patches are smaller in size in the gE deletion mutant-infected cells, but the virion number in those patches is increased at 12 hpi. (B) Quantitative determination of the percentage of adherent cell membrane covered by glycoprotein patches and (C) the amount of virions on the total adherent membrane. Quantifications support the visual data. (D) Infected cells were harvested at 10 hpi, and the titer was determined by limiting-dilution plaque assay. Note that gE deletions had little effect on the production of progeny virus titer despite the observation that the number of virions on the adherent surface was increased. P values of deletion mutants compared to rescue virus are labeled the following: *, <0.05; **, <0.005; and ***, <0.0005. Bars represent standard errors of the means.