Fig 4.

Caspase activity present in gut extracts collected from third-instar C. zealandica larvae at 0, 16, 24, 36, or 48 h after control or Yen-Tc treatment (as indicated). (A) Hydrolysis of the caspase-3/7 substrate (DEVDpNA at a 0.1 mM final concentration) measured after incubation in either the absence or the presence of the caspase-3/7 inhibitor (DEVD-CHO at a 0.1 mM final concentration). (B) Hydrolysis of the caspase-3/7 substrate (DEVDpNA) measured after incubation in either the absence or the presence of the serine protease inhibitors leupeptin and aprotinin (0.1 mM each) (+Leu+Apro). The cleavage of DEVDpNA was measured as the release of p-nitroaniline (pNA).