Table 2.
Frequency of detection of Sps colonies during the culture of first-crossover integrantsa
| Addition of pTBR101-CM to reaction mixture for electroporation | No. of tested colonies (A) | No. of Sps second-crossover recombinants (B) | Detection frequency (%) |
|---|---|---|---|
| 105-A βgal:: pBS423-ΔrepAβgal | |||
| − | 2,396 | 3 | 0.125 |
| + | 3,568 | 3,568 | 100 |
| 105-A aga:: pBS423-ΔrepAΔaga | |||
| − | 963 | 1 | 0.104 |
| + | 1,645 | 1,643 | 99.9 |
a
The reaction mixtures were supplemented with pTBR101-CM for electroporation (+) or left unsupplemented (−). Detection frequency was determined by the following equation: (number of Sps second-crossover recombinants/number of tested colonies) × 100.