Fig 3.

Relative expression of the genes involved in the arginine deiminase pathway of Lactobacillus sakei CTC 494 when grown in modified MRS medium supplemented with 3 g liter⁻¹ (17.2 mM) of arginine as a function of the growth phase (late exponential [EXP.; OD₆₀₀, 0.3], end exponential [OD₆₀₀, 0.5], early stationary [STAT.; OD₆₀₀, 0.9], and late stationary [OD₆₀₀, 1.0 after 24 h of fermentation] growth phases) and environmental pH (pH 5.0, pH 6.0, and pH 7.0, indicated from left to right, respectively, for each set of bars): arcA (A), arcB (B), arcC (C), arcT (D), arcD (E), and arcR (F). The data shown are mean values ± standard deviations from four independent fermentation experiments, whereby the probability of expression levels different from those in the control fermentation was calculated by the pairwise fixed reallocation randomization test (50,000 permutations) between samples and control groups (P < 0.001).