Table 1.
Purification of GH78 protein from X. polymorpha (measured by demethylation of methyl ferulate) produced during solid-state fermentation of wheat strawa
| Purification step | Total activity (U) | Total protein (mg) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Wheat straw extraction | 57.4 | 910.8 | 0.06 | 100 | 1.0 |
| Ultrafiltration | 54.5 | 620.5 | 0.09 | 94.9 | 1.5 |
| DEAE-Sepharose separation | 43.9 | 68.2 | 0.64 | 76.5 | 10.7 |
| Superdex Sepharose SEC | 20.6 | 11.5 | 1.79 | 35.9 | 29.8 |
| Mono Q column separation | 4.9 | 1.9 | 2.58 | 8.5 | 43.0 |
| Biosep-SEC-S-2000 column separation | 3.8 | 1.7 | 2.24 | 6.6 | 37.3 |
a
Cultures were harvested after 25 days and extracted with water.