Fig 4.
Identification of phospholipase A1 activity of MPlaG. Positional specificity of MPlaG toward OPPC (1-oleoyl-2-palmitoyl-phosphatidylcholine) was determined by LC-MS. (A) The reaction products of MPlaG were separated by HPLC at the indicated retention times, i.e., 16.93 min (m/z 804; OPPC) (upper part of panel B) and 18.14 min (m/z 540; 2-palmitoyl-lysophosphatidylcholine) (lower part of panel B). Gradient elution was performed as follows: 0 to 25 min, 2 to 38% mobile phase A (linear gradient); and 25 to 35 min, 70% mobile phase A (isocratic).