Fig 2.

Multiple sequence alignment of conserved domains in MpaDE and selected orthologs. The P450 heme signature motif, the EXXR motif of P450 heme thiolate enzymes, and the Zn-hydrolase motif HXHXDH are boxed. Conserved residues are written in red in the alignment. MpaD orthologs: PDB code 2ij2 (Bacillus megaterium P450 BM3), PDB code 3k9v (Rattus norvegicus CYP24A1), PDB code 2f9q (Homo sapiens CYP2D6), TSTA_060710 (Talaromyces stipitatus TSTA_060710), SNOG_06679 (Phaeosphaeria nodorum SNOG_06679). MpaE orthologs: PBD code 3dha (Bacillus thuringiensis lactone hydrolase), PBD code 2r2d (Agrobacterium tumefaciens lactone hydrolase), PBD code 1sml (Stenotrophomonas maltophilia lactone hydrolase), TSTA_060680 (Talaromyces stipitatus TSTA_060680), SNOG_06681 (Phaeosphaeria nodorum SNOG_06681).