MpaDE catalyzes the conversion of 5-MOA to DHMP. UHPLC-UV/VIS-HRMS analyses of the standards 5-MOA and DHMP and A. nidulans strains NID211, NID410, and NID416. (A) Extracted ion chromatogram, m/z 183.06519 ± 0.001 corresponding to the [M+H]+ ion of 5-methylorsellinic acid. The MS spectrum at 4.0 min is inserted in relevant chromatograms. (B) Extracted ion chromatogram (m/z 181.04954 ± 0.001) corresponding to the [M+H]+ ion of DHMP. The MS spectrum at 2.9 min is inserted in relevant chromatograms. 3-MOA, 3-methylorsellinic acid; 5-MOA, 5-methylorsellinic acid; DHMP, 5,7-dihydroxy-4-methylphthalide.