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Cloning and expression of gshF genes from three different Gram-positive bacteria in P. pastoris GS115. (A) PCR amplification of the three gshF genes. Lanes M, molecular size markers; lane 1, L. monocytogenes gshF; lane 2, S. agalactiae gshF; lane 3, L. plantarum gshF. (B) Schematic representation of the construction of a recombinant expression vector containing the gshF genes derived from Gram-positive bacteria.
