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Evaluation of GSH synthesis capability of recombinant P. pastoris strains expressing L. monocytogenes gshF and S. cerevisiae GSHI/GSHII with and without feeding with three amino acid precursors. A, without amino acid precursors; B, with glycine, glutamate, and cysteine. Each amino acid at 5 mM was fed to the flask 1 h before the harvesting of cells.
