Table 1.

PCR-related information for cloning of gshF genes

Gene name	Source	Primer pair used for amplificationa	Gene size (bp)	Closest homologue(s) (% homology)
Lm-gshF	L. monocytogenes	LmgshF-F, 5′-AGATCGATACCATGATAAAACTTGATATGAC-3′; LmgshF-R, 5′-CAGCGGCCGCTTAGTCAAATAAGAAATCTAA-3′	2,346	L. monocytogenes M7, L99, HCC23 (99)
Sa-gshF	S. agalactiae	SagshF-F, 5′-AGATCGATACCATGATTATAGATCGACTGTTACAAAGAAGCCACTCTCATCTAC-3′; SagshF-R, 5′-CTAGCGGCCGCTTAGCCTAAGGAACTCTTTTTAGTTA-3′	2,276	S. agalactiae NEM316, 2603V/R, A909 (100)
Lp-gshF	L. plantarum	LpgshF-F, 5′-AGATCGATACCATGGAATTAGATGCCGTTGGTA-3′; LpgshF-R, 5′-CAGCGGCCGCTTAATCTTCATTTTTAAACAATGC-3′	2,256	L. plantarum WCFS1 (100)

^aClaI and NotI sites are underlined.