Table 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | TaKaRa | |
| P. pastoris GS115 | his4− Mut+ | Invitrogen |
| S. cerevisiae S288c | Our lab | |
| L. monocytogenes | Our lab | |
| S. agalactiae | Our lab | |
| L. plantarum | Our lab | |
| GS115/LmgshF | GS115 integrated with recombinant plasmid pGAPZH-LmgshF | This study |
| GS115/SagshF | GS115 integrated with recombinant plasmid pGAPZH-SagshF | This study |
| GS115/LpgshF | GS115 integrated with recombinant plasmid pGAPZH-LpgshF | This study |
| GS115/Sc288c | GS115 integrated with recombinant plasmid pGAPZH-ScGSH | This study |
| Plasmids | ||
| pGAPZαA | Vector for constitutive secreted protein expression; Zeor | Invitrogen |
| pPIC9k | Vector for inducible secreted protein expression; HIS4; Kanr Ampr | Invitrogen |
| pGAPZαAH | pGAPZαA-based expression vector carrying HIS4 fragment from pPIC9k; Zeor | This study |
| pGAPZH-Scgsh1 | pGAPZαAH containing gsh1 from S. cerevisiae S288c; Zeor | This study |
| pGAPZH-Scgsh2 | pGAPZαA containing gsh2 from S. cerevisiae S288c; Zeor | This study |
| pGAPZH-ScGSH | pGAPZαAH containing gsh1 and gsh2 from S. cerevisiae S288c; Zeor | This study |
| pGAPZH-LmgshF | pGAPZαAH containing gshF from L. monocytogenes; Zeor | This study |
| pGAPZH-LpgshF | pGAPZαAH containing gshF from L. plantarum; Zeor | This study |
| pGAPZH-SagshF | pGAPZαAH containing gshF from S. agalactiae; Zeor | This study |