Fig 1.

Construction of lacZ reporter strains. The lacZ reporter cassette was integrated into the Tmku80 locus by ATMT and confirmed by Southern blotting. (A) Schematic representation of homologous recombination. Restriction sites and sizes of expected fragments on Southern blotting are shown. “Promoter” represents either P_CTR4 or P_CRP1. B, BamHI; P, PstI; Hyg^r, hygromycin B resistance cassette; L and R arms, homologous sequences for recombination at the Tmku80 locus; LB and RB, left and right borders, respectively. (B) Total DNA from each strain was digested with PstI and separated by gel electrophoresis. Southern blotting hybridization was performed as described in Materials and Methods. A partial fragment of Tmku80 393 bp in length was used as a hybridization probe.