Table 2.
Primers used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| PCTR4-F/BamHI | ATGGATCCTCGTAGGTACGCC |
| PCTR4-R | ATGAATTCTTCGCAGGCTTTGTAC |
| PCRP1-F/BamHI | ATGGATCCGGAACTCGTTCTTCCATATC |
| PCRP1-R | ATGAATTCGGTGATAGACGGTTCAGTG |
| lacZ/PCTR4-R | AGCCGTTCATTTCGCAGGCTTTGTACTTTG |
| PCTR4/lacZ-F | AGCCTGCGAAATGAACGGCTCCGGAGCTTG |
| TtrpC/lacZ-R | GAGACCCGGGTTTTTGACACCAGACCAAC |
| lacZ/TtrpC-F | GTGTCAAAAACCCGGGTCTCGAGCAGCTAG |
| TtrpC-R/BamHI | TCGGGATCCAAAGAAGGATTACCTCTAAAC |
| PCTR4-F/KpnI | GCATGGTACCTCGTAGGTACGCCCTTCCGG |
| ERG1R/PCTR4-R | ACCATTGTAGTTCGCAGGCTTTGTACTTTG |
| PCTR4/ERG1R-F | GCCTGCGAACTACAATGGTTGTAGAGGCTC |
| ERG1R-R/SacI | GTTTGAGCTCATTATCTGGCCACCTTTGTT |
| ERG1L-F/SpeI | TCCAACTAGTGGAATTAGATAAATCTGGCC |
| ERG1L-R/SpeI | CCATACTAGTAACTTGTCGAAGGGGATAAA |
| TRP5R-F | CAATGGAGCAAATAAAGGAGAC |
| TRP5R-R | TGAATCCAATTAGGGCCTCTG |
| TRP5L-F | GAAAATCATTGGGAAGTATTC |
| TRP5L-R | TGAATGGTGTTGTATATGTTGTGTCG |
a
Restriction recognition sites are underlined.