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. 2012 Aug;78(15):5035–5042. doi: 10.1128/AEM.00551-12

Table 2.

Plasmids used for recombinant protein expression in bifidobacteria

Plasmid Replicon Promoter Selection markera Protein expressed Host species Localization signal Reference
pBLES100-S-eCD pTB6 Phup Spc E. coli cytosine deaminase B. longum 43
pAV001-HU-eCD pTB6 Phup Spc E. coli cytosine deaminase B. breve, B. longum 21
pAV001-HU-eCD-M968 pTB6 Phup Spc E. coli cytosine deaminase B. longum 20
pBLES-FliC pTB6 Phup Spc S. typhimurium FliC B. animalis 68
pJW245 pTB6 Phup Spc S. typhimurium FliC B. longum GltA-FliC fusion for surface display 75
pBV220/endostatin pMB1 λPRPL Amp Human endostatin B. adolescentis, B. longum 15, 35
pBV22210 pMB1 λPRPL Cm Human endostatin B. longum 74
pBV22210-TRAIL pMB1 λPRPL Cm Human TNF-related apoptosis-inducing ligand B. longum 22
pLR2 pMB1 Phup Cm Synthetic human interleukin 10 B. longum β-Galactosidase signal peptide 56
pBIFRIBO-gusA pASV479 P16SrRNA Cm β-Glucuronidase B. breve 64
pESH86 pB80 Phup Erm Human fibroblast growth factor B. breve Sec2 signal peptide 66
pESH46 pB44 Phup
pESH47 pB44 Pgap
pGUSA pNCC293 Pgap Spc E. coli β-glucuronidase B. longum 30
pGUSB pNCC293 PBL1613 Spc
pGUSC pNCC293 Paga Spc
pPSAB1 pMG1 Pamy Cm Pediococcus spp. pediocin PA-1 B. longum α-Amylase signal peptide 42
pBES16PR-CHOL pMG1 P16SrRNA Cm S. coelicolor cholesterol oxidase B. longum 50
pESH92 pB80 Phup Erm Human interleukin 10 B. breve Sec2 signal peptide 27
pESH93 pB80 Pgap Erm Human interleukin 10 B. breve Sec2 signal peptide
pESH99 pB80 Pgap Erm Human interleukin 10 B. breve Sec2 signal peptide
pESH100 pB80 Pgap Erm Human interleukin 10 B. breve AmyB signal sequence
pESH101 pB80 Pgap Erm Human interleukin 10 B. breve ApuB signal sequence
pESH102 pB80 Pgap Erm Human interleukin 10 B. breve Sec2 signal peptide
pESH103 pB80 Pgap Erm Human interleukin 10 B. breve Sec2 signal peptide
pLuxMC2 pBC1 Prep Cm Photorhabdus luminescensluciferase B. breve 10
pLuxMC3 pBC1 Phelp Cm P. luminescens luciferase B. breve
pBEX–CfaB pBC1 Pamy Amp Enterotoxigenic E. coli CfaB B. infantis 37
pBEX–LTB pBC1 Pamy Amp Enterotoxigenic E. coli LTB B. infantis
pMDYP469AbfB pNCC293 PbetA Cm B. longum arabinofuranosidase B. longum, B. breve, B. adolescentis, B. pseudocatenulatum Bile inducible 62
pGBL8b pNAL8L T5 Cm Firefly luciferase B. longum 19
pBBADs-OXM ? ParaC Amp Human oxyntomodulin B. longum XynF signal peptide 36
pBBADs-IL-10 ? ParaC Amp Human interleukin 10 B. longum XynF signal peptide 76
pBBADs-IL-12 ? ParaC Amp Human interleukin 12 B. longum XynF signal peptide 79
a

Spc, spectinomycin; Amp, ampicillin; Cm, chloramphenicol; Erm, erythromycin.