TABLE 2.
Substrate | Km (mM) | kcat (s−1) | kcat/Km (s−1 · mM−1) |
---|---|---|---|
l-Gln | 5.22 ± 0.10 | 489 ± 103 | 93 ± 21 |
l-Gly-Gln | 0.66 ± 0.12 | 711 ± 84 | 1085 ± 74 |
l-Leu-Gly-Gln | 0.43 ± 0.13 | 751 ± 67 | 1870 ± 666 |
l-Pro-Leu-Gly-Gln | 0.33 ± 0.08 | 833 ± 20 | 2662 ± 786 |
Cbz-Gln | 0.07 ± 0.02 | 833 ± 63 | 11986 ± 3014 |
l-Asn | 8.03 ± 0.18 | 1023 ± 39 | 127 ± 7.0 |
l-Gly-Asn | 0.63 ± 0.03 | 439 ± 46 | 692 ± 36 |
l-Leu-Gly-Asn | 1.06 ± 0.06 | 408 ± 56 | 388 ± 71 |
l-Pro-Leu-Gly-Asn | 0.74 ± 0.19 | 715 ± 77 | 991 ± 144 |
Assays were carried out in triplicate and the results represent the means ± standard deviations.