Skip to main content
. 2012 Aug;78(15):5182–5188. doi: 10.1128/AEM.00765-12

TABLE 2.

Kinetic properties of AsGahAa

Substrate Km (mM) kcat (s−1) kcat/Km (s−1 · mM−1)
l-Gln 5.22 ± 0.10 489 ± 103 93 ± 21
l-Gly-Gln 0.66 ± 0.12 711 ± 84 1085 ± 74
l-Leu-Gly-Gln 0.43 ± 0.13 751 ± 67 1870 ± 666
l-Pro-Leu-Gly-Gln 0.33 ± 0.08 833 ± 20 2662 ± 786
Cbz-Gln 0.07 ± 0.02 833 ± 63 11986 ± 3014
l-Asn 8.03 ± 0.18 1023 ± 39 127 ± 7.0
l-Gly-Asn 0.63 ± 0.03 439 ± 46 692 ± 36
l-Leu-Gly-Asn 1.06 ± 0.06 408 ± 56 388 ± 71
l-Pro-Leu-Gly-Asn 0.74 ± 0.19 715 ± 77 991 ± 144
a

Assays were carried out in triplicate and the results represent the means ± standard deviations.