Table 3.
Comparison of C. difficile counts by RT-qPCR, qPCR, and the anaerobic culture method
| Group and subjecta | Log10 cell count or CFU/g of feces |
Δ value |
|||
|---|---|---|---|---|---|
| RT-qPCR | qPCR | Culture | RT-qPCR versus qPCR | RT-qPCR versus culture | |
| A-1 (facility residents) | |||||
| SO-5 | 6.4 | 6.7 | 5.0 | −0.3 | 1.3 |
| SO-13 | 2.0 | ||||
| SO-14 | 2.2 | ||||
| SO-15 | 2.4 | ||||
| SO-16 | 6.3 | 6.6 | 4.0 | −0.2 | 2.3 |
| SO-19 | 5.3 | 5.5 | 4.4 | −0.3 | 0.9 |
| SO-21 | 5.8 | 6.5 | 4.8 | −0.7 | 1.0 |
| SO-22 | 2.4 | ||||
| SO-23 | 5.8 | 6.4 | 5.5 | −0.6 | 0.3 |
| SO-24 | 2.4 | ||||
| SO-25 | 6.4 | 6.9 | 6.0 | −0.5 | 0.4 |
| SO-26 | 5.2 | 5.9 | 3.4 | −0.7 | 1.8 |
| SO-28 | 4.4 | 5.1 | 3.8 | −0.7 | 0.6 |
| SO-29 | 2.0 | ||||
| SO-31 | 5.8 | 5.9 | 4.8 | −0.1 | 1.0 |
| SO-32 | 2.5 | ||||
| SO-37 | 7.8 | 8.8 | 4.6 | −0.9 | 3.2 |
| SO-45 | 6.0 | 6.5 | 5.8 | −0.4 | 0.3 |
| SO-48 | 2.0 | ||||
| SO-49 | 2.6 | ||||
| SO-50 | 2.0 | ||||
| SO-53 | 2.0 | ||||
| SO-55 | 5.5 | 6.0 | −0.5 | ||
| SO-57 | 2.1 | ||||
| SO-58 | 7.0 | 7.8 | 4.4 | −0.8 | 2.6 |
| SO-60 | 6.9 | 7.6 | 4.7 | −0.6 | 2.2 |
| SO-62 | 3.5 | ||||
| SO-64 | 2.2 | ||||
| SO-69 | 3.1 | ||||
| SO-70 | 2.5 | ||||
| SO-73 | 2.3 | ||||
| SO-75 | 2.1 | ||||
| SO-87 | 7.2 | 7.9 | 6.3 | −0.7 | 0.9 |
| SO-93 | 2.2 | ||||
| SO-98 | 2.0 | ||||
| SO-99 | 5.9 | 6.4 | 6.6 | −0.4 | −0.6 |
| A-2 (facility staff) | |||||
| SO-100 | 2.3 | 2.9 | −0.6 | ||
| SO-113 | 2.1 | ||||
| SO-120 | 2.3 | ||||
a
Subjects who tested positive for C. difficile are listed.