FIG 1.

Summary of on-line and off-line analyses from fed-batch methanol cultivation of PB1 (A) and MGA3 (B) in UMN1 medium. CO₂, CO₂ evolution rate as a function of time (mmol liter⁻¹ h⁻¹); CDW, dry weight (g liter⁻¹) of cells (filled circles), calculated from OD₆₀₀ measurements by correlation to selected measurements of dry weights of cells; Glu, glutamate concentration in the medium (mmol liter⁻¹) (filled triangles); Lys, lysine concentration in the medium (mmol liter⁻¹) (filled inverted triangles); MeOH, methanol concentration (mmol liter⁻¹), determined by in-line mass spectrometric measurement in the exhaust gas and automatically held constant at 150 mmol liter⁻¹; DO, dissolved oxygen (%). Values for CO₂, dry weight of cells, Glu, and Lys are corrected for volume changes during cultivation.