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Mechanosensitive channel activity of wild-type NCgl1221 (left) and NCgl1221 (A111V) (right) in E. coli strain MJF612 (ΔmscM ΔmscS ΔmscK ΔmscL). The membrane current and its vertical enlargement and pressure are shown from the top to the bottom. Upward and downward arrowheads represent the start and end of the channel activity of NCgl1221, respectively. All experiments were performed at a holding potential of −20 mV.
