Fig 4.

Distribution of putative proto-spacers on plasmids PMA1 (A), pMA1 (B), and pMA2 (C). Each bar indicates the position of the putative proto-spacer. Solid and dashed bars indicate putative proto-spacers in clockwise and counterclockwise strands, respectively. An asterisk indicates 100% nucleotide identity. Of note, we found additional ORFs in pMA1 (ORF b) and pMA2 (ORFs a, b, e, g, and h) compared with their original description. ORF a and b of pMA1 may be generated from a longer replication gene by a frameshift. ORFs g, h, a, b, and c may be generated by frameshifts from a single hypothetical gene whose homologues are prevalent in cyanobacteria (e.g., CY0110_05002 of Cyanothece sp. strain CCY0110).