Table 3.
Spacers showing silent or conservative mutations compared to Ma-LMM01 putative proto-spacers
| Spacer/phage genea | Nucleotide sequencec | Predicted amino acid sequencec |
|---|---|---|
| 298spc124b | AGTGGCGCGGCTACTTATCTCTACCAATTTTCTAC | SGAATYLYQFS |
| ORF40 | ........A..C..............GG....C.. | .........V. |
| 7806spc34b | ATTTGAGGGACTAAATAATGGGATCGTATTCAAT | FEGLNNGIVFN |
| ORF41 | .........CGC...C................G. | ...A......S |
| CT6spc2b | AATCCCCCCGTCAGGGATTCTCCCACGGGTTTCAAT | IPPSGILPRVS |
| ORF61 | ...A...........AG................T.. | .....V..... |
| CT15spc12b | ACTCTCCTTGCGACTATAAGTATGTGGGCAAGTCT | SPCDYKYVGKS |
| ORF25 | ............................T...... | ........... |
| CT15spc13b | TCTATCTGTTCAATACTATGCCTCTAGGAGCAGGGCAAG | YLFNTMPLGAGQ |
| ORF20 | .....................................G. | ............ |
| CT15spc14b | TTGATACAGGTGCCTTCCTAGGCTGTTATCT | DTGAFLGCY |
| ORF25 | .......G....................... | ......... |
a
In each row, the spacer (top) and the corresponding putative proto-spacer (bottom) are shown.
b
Reverse complementary sequences are shown.
c
Identical nucleotides and amino acids are indicated by dots. Synonymous and conservative changes are in bold and italic letters, respectively. The seed region identified in the subtype I-E CRISPR of E. coli (43) is underlined.