. 2012 Jul;80(7):2538–2547. doi: 10.1128/IAI.06354-11

Table 1.

Primers used for cloning

Primer	Sequence (5′–3′)
uhs_pilU_KO_f	`ATGCCGTCTGAAATGCTGTCCGAATCGCTGACC`
uhs_pilU_KO_r	`GTATGGGGCTGCGTTAGCTTCTTTTCGGTTT`
dhs_pilU_KO_f	`GACCTGAATGGAATGATCCGCAAAACCCAATGCC`
dhs_pilU_KO_r	`ACCGCGCCTAAAATGGTAAAGC`
tetA_pilU_KO_f	`GAAGCTAACGCAGCCCCATACGATATAAGTTG`
tetA_pilU_KO_r	`GCGGATCATTCCATTCAGGTCGAGGTGGC`
uhs_pilUC_f	`TTGCATCCTGCTGTATAAAACC`
uhs_pilUC_r	`TCTTCCGTCAGCTATGGATGGCGGCGTTT`
dhs_pilUC_f	`CATTTCCTGAGCATTGGTAAAGCACGCGGGGAACGGAAA`
dhs_PilUC_r	`AGGTAGGCGGAAACGAGGGTA`
pilU_pilUC_f	`TAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAGGATCCTATGAATACCGATAACCTGCACG`
pilU_pilUC_r	`TTACCAATGCTCAGGAAATGAGGTTGAGACCG`
cat_pilUC_f	`CATCCATAGCTGACGGAAGATCACTTCGCA`
cat_pilUC_r	`GCTCACAATTCCACACATTATACGAGCCGATGATTAATTTTCAACAGCTC`