Fig 2.

p38 and ERK1/2 signaling pathways are involved in the secretion of KC and MCP-1 by B. abortus-infected primary osteoblasts. Effects of SB23850 (SB), a p38 MAPK inhibitor, and PD98059 (PD), an ERK1/2 inhibitor, on B. abortus induction of MCP-1 (A) and KC (B), respectively, are shown. Levels of MCP-1 and KC production by primary mouse osteoblasts were measured at 48 h after infection with B. abortus at an MOI of 100 by ELISA. P values for comparison with results for untreated infected cells (Untreated) are as follows: **, P < 0.01; ***, P < 0.001. Data shown are from a representative experiment of five performed.