Fig 1.

Adhesion and invasion assays using Caco-2 cells confirm the role of cj1136 in host epithelial cell invasion but not adhesion. The adhesion phenotypes of C. jejuni wild-type (WT) 01/51 and C. jejuni 01/51 Δcj1136 (ΔCj1136) (A) and the invasion phenotypes of C. jejuni WT 01/51, C. jejuni 01/51 Δcj1136, and C. jejuni 01/51 Δcj1136::cj1136 (1136 Comp) (B) were determined using Caco-2 cells. A double mutant of C. jejuni 81116 with mutations in flaA and flaB (ΔflaA/B) was used as a negative control in both assays. A paired Student t test was performed to compare the invasion levels of (i) the mutant (ΔCj1136) and negative control (ΔflaA/B) with that of the WT strain and (ii) the complemented mutant (1136 Comp) with that of the mutant (ΔCj1136). **, P < 0.001. As there was no change in adhesion between the wild type and mutant, the complemented mutant was not tested in this assay.