Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| C. jejuni | ||
| 01/51 | Hyperinvasive wild-type strain 01/51 | 11 |
| 01/51 Δcj1136 | cj1136 mutation generated in strain 01/51 | This study |
| 01/51 Δcj1136::cj1136 | cj1136 mutation complemented by wild-type copy of the gene in strain 01/51 | This study |
| 81116 Δ(flaA flaB) | Double mutation in flaA and flaB genes in strain 81116 | 52 |
| E. coli TOP10F′ | F′ lacIq Tn10(Tetr) mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| Plasmids | ||
| pGEM-T Easy | Cloning vector encoding resistance to ampicillin | Promega |
| pGEM:1136::kan | pGEM-T Easy vector carrying 1.3-kb fragment of cj1136 interrupted by Kanr cassette | This study |
| pAJ20 | 2.3-kb DNA fragment flanking the intergenic region between cj0652 and cj0653c with additional BamHI restriction site in the intergenic region, cloned into pGEM-T Easy vector | This study |
| pAJ22 | Chloramphenicol resistance cassette cloned into pAJ20 | This study |
| pAJ22-1136 | Wild-type copy of cj1136 with its own promoter cloned into pAJ22 vector | This study |
| pMA24 | Source of kanamycin resistance cassette | 1 |
| pUOA18 | Source of chloramphenicol resistance cassette | 51 |