Table 2.
Primers used in this study
| Primer | Nucleotide sequence (5′ → 3′)a |
|---|---|
| 1136-F | GCTCGAAATCAATCATCAAATTT |
| 1136-R | CAACTCTTTGGGAAGAAATTCAA |
| 1136-INF | caaggatccGCACTATCACTCCAAGAGACTATG |
| 1136-INR | caaggatccCCATCATCATAAATACAAGCATG |
| Kan-F | ggaggatccGATAAACCCAGCGAACC |
| Kan-R | gcaggatccGACATCTAAATCTAGGTACTAAAACAATTC |
| C1136-F | caaggatccCGCAAAGTAATTTTACAAGGTTCA |
| C1136-R | caaggatcctCAACTCTTTGGGAAGAAATTCAA |
| 652-F | GAACTTACGATAGATATAGAGC |
| 653-F | TTATGATGAAATCATCATGGAGC |
| IN652-R | caaggatccCAAGATTTTTAAACCAATTGC |
| IN653-R | caaggatccAATTATAAGTTTAATACAAGG |
| CML-F | caaggatccGTCGGTATCGTATGGAGCG |
| CML-R | cacagatctCCTAAAGGGTTTTTATCAGTGCG |
a
BamHI (ggatcc) or BglII (agatct) restriction sites and additional nucleotides at 5′ ends of primers, where needed, are shown in lowercase.