Table 1.
VPO1-mediated formation of Cl-Tyr detected by mass spectrometrya
| Sample | Cl-Tyr concn (μM) | Cl-Tyr/Tyr (%) |
|---|---|---|
| NaCl + H2O2 | 0 | 0 |
| H2O2 + VPO1 | 0 | 0 |
| NaCl + H2O2 + VPO1 | 0.0162 | 0.0162 |
| HOCl | 0.0275 | 0.0275 |
a
140 mM NaCl, 500 μM H2O2, and/or 1,000 μM VPO1 were added to 50 mM phosphate buffer (pH 5.5) containing 100 μM Tyr. The mixture was incubated at 37°C overnight. HOCl (50 μM) was added into 100 μM Tyr solution in 50 mM phosphate buffer (pH 5.5) as a positive control. The sample (10 μl) was analyzed by LC-MS. The Cl-Tyr concentration was calculated from the Cl-Tyr standard curve.